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Dope on standard material: The established might be recombinant protein or natural protein, that will depend on the specific kit. Furthermore, the expression system is E. And the wash solution is TBS.
The standard diluent contains 0. Some kits can curb is BSA in them. The provided antibodies and their emcee vary in different kits. Usual the duplicate readings for each standard, control, and samples and subtract the average zero regulatory optical density.
Construct a criterion curve by plotting the capital O.
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It will not have any effect on the final assay results.
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